Alternative HealthGrand Forks

Men's health & testosterone

How to Prepare for a Testosterone Blood Test and Get a Clear Result

Timing, sleep, fasting and illness can all shift a testosterone result. Here is how to prepare so your numbers are easier to interpret.

Key takeaways
  • Testosterone is usually highest in the morning, so tests are drawn early in the day.
  • Guidelines call for two morning tests on separate days before a diagnosis.
  • Food, acute illness, poor sleep and some medicines can lower a reading.
  • Take your usual medicines unless your clinician tells you otherwise.
  • The clinic can give you a lab order by phone, and the lab bills for the tests separately.

A testosterone result can steer big decisions, so it is worth getting a reading you can trust. A draw at the wrong time of day, after a short night or while you are fighting off a cold can make your level look lower than it usually is. A little planning makes your results much easier to read.

Why Morning Timing Matters

Testosterone rises and falls over the day. The Endocrine Society guideline notes that levels peak in the morning. MedlinePlus says the blood sample is usually taken between 7 and 10 a.m., when levels are typically highest.

An afternoon result may come back lower simply because of the clock. That is why the American Urological Association (AUA) guideline calls for testing in the early morning.

If you work nights or rotating shifts, "morning" can be hard to pin down. Tell your clinician your schedule before you book, and ask how to time your draw.

Why You Need Two Tests

The AUA guideline says low testosterone should be diagnosed only after two total testosterone tests taken on separate occasions, both in the early morning. A diagnosis also needs symptoms or signs, not just a number.

One test is not enough because levels vary from day to day. The Endocrine Society guideline reports that 30% of men with an initial result in the low range have a normal level when the test is repeated. A second test helps sort out a pattern from a one-off dip.

How to Prepare, Step by Step

  1. Get your lab orderAlternative Health Grand Forks can give you a lab order by phone. Call (218) 274-4500 and Will can help. The lab bills for the tests separately.
  2. Book an early drawChoose the earliest slot you can, ideally between 7 and 10 a.m. If you work nights, ask your clinician how to time it first.
  3. Sleep as normally as you canAim for a regular night of sleep before the test. Avoid booking the morning after a night shift or a very short night.
  4. Fast if the lab asksThe Endocrine Society guideline describes testing while fasting, because food and sugar can lower testosterone. Follow the fasting instructions from your clinician or the lab, and ask whether water is fine.
  5. Take your usual medicinesMedlinePlus advises telling your provider about everything you take and not stopping any medicine unless your provider tells you to.
  6. Reschedule if you are sickIf you have a fever, the flu or another acute illness, call and move the test. Rescheduling is free.
  7. Repeat on another dayBook the second test on a different morning, with the same preparation, so the two results can be compared fairly.

What Can Throw off a Result

Some things lower a reading for a while without meaning your usual level is low. The Endocrine Society guideline says men should not be tested while they have or are recovering from an acute illness, or while taking short-term medicines that suppress testosterone, such as opioid pain medicines.

  • Illness. A recent infection, surgery or hospital stay can lower testosterone for a while.
  • Short sleep. In a small study of 10 healthy young men, one week of sleeping about 5 hours a night lowered daytime testosterone. The authors called for more research, but it is one more reason to test after normal sleep.
  • Food. Eating or drinking sugar before the draw may lower the result, which is why fasting is often requested.
  • Some medicines. The Endocrine Society lists opioids and glucocorticoids (steroid medicines) among causes of low testosterone. Testosterone products, anabolic steroids and some supplements can also change results.
  • Health conditions. Obesity, diabetes and sleep disorders such as sleep apnea affect testosterone levels too. These are not errors; they are part of the picture your clinician will look at.

Other Labs Often Ordered with It

Your clinician may order other tests on the same draw or after the first results. The AUA guideline describes several:

  • Luteinizing hormone (LH) for men with low testosterone, to help find where the problem starts. The Endocrine Society also recommends follicle-stimulating hormone (FSH).
  • Prolactin when testosterone is low and LH is low or normal.
  • Hemoglobin and hematocrit, part of a blood count, before any testosterone treatment.
  • PSA (prostate-specific antigen) for men over 40 before treatment.
  • Estradiol if you have breast tenderness or swelling.

The Endocrine Society guideline also describes measuring free testosterone in some men, such as those with obesity or diabetes, whose binding proteins may be affected.

What to Bring to Your Consultation

  • Copies of any past testosterone results, with the date and time of day each was drawn
  • A list of all your medicines and supplements, with doses
  • Any testosterone, anabolic steroid or hormone products you use now or have used
  • Your work and sleep schedule, especially if you work nights or rotating shifts
  • Notes on your symptoms and when they started
  • Any recent illness, surgery or hospital stay
  • Whether you might want children in the future

If your past results are with another clinic, the office can explain how to have them sent. Records can be faxed to (701) 757-0974, or downloaded from your patient portal and emailed to info@alternativehealthgf.com.

Next Steps

To get a lab order or book a visit, see our testosterone consultation page or contact the office. If you may want children, read testosterone therapy and fertility first, and for the bigger picture, see what a low testosterone evaluation can and cannot tell you.

Sources

  1. American Urological Association: Testosterone Deficiency Guideline
  2. Endocrine Society (Bhasin et al., J Clin Endocrinol Metab 2018): Testosterone Therapy in Men With Hypogonadism
  3. MedlinePlus: Testosterone Levels Test
  4. Leproult, Van Cauter. JAMA 2011: Effect of 1 week of sleep restriction on testosterone levels in young healthy men
About this guide
This guide is general information, not medical advice for your situation. Medical disclaimer

Questions

Related Questions

Short answers on the same topic as this guide. Still unsure? Call Will at (218) 274-4500.

See every question
How much does TRT cost at Alternative Health?
  • Initial consultation: $200. If you qualify and decide to start treatment, it includes your first 4-week supply of testosterone, supplies and shipping.
  • Every 4 weeks after that: $175, OR
  • Pay $2,000 upfront for the whole year and save $300. ($200 consultation fee is credited toward membership, leaving a balance of $1,800)
  • Follow-up visits are included in both plans.
  • Lab work is billed separately by the lab you choose. (If you don't have a preference, we can refer you to a lab.)

Request a Consultation

Do I need lab work before a testosterone consultation?

Usually, yes. Call us for a lab order, complete the labs, and then book your visit so Dr. Ewals can review the results with you. Low testosterone is generally confirmed with early-morning blood tests on more than one day, not symptoms alone.

What are the common signs of low testosterone?

Low energy, low libido or weaker erections, losing muscle and gaining weight around the midsection, low mood, poor concentration and poor sleep. Every one of these has other possible causes, including sleep apnea, thyroid problems, medicines and stress, which is why we test rather than guess.

What does treatment involve if my levels are low?

Dr. Ewals sets a dose and shows you how the medicine is taken. Your medication and home-use supplies are shipped to you as part of the TRT membership. Follow-up visits are every 8 to 24 weeks, depending on the person, and your dose is adjusted from your lab results.

How often will I need labs on treatment?

Once your levels are stable, usually after 2 to 4 months, labs are typically every 3 to 6 months. They check testosterone, red blood cell count and other markers. Labs are billed by the lab and aren't included in the TRT membership.

What did the FDA change about testosterone in 2025?

Two things. After a large trial (TRAVERSE) found no increase in major heart problems, the FDA had testosterone labels updated to reflect those results. It also required a warning that testosterone can raise blood pressure, so Dr. Ewals monitors yours.

Who shouldn't take testosterone?

Men with or being evaluated for prostate or breast cancer, untreated severe sleep apnea, uncontrolled heart failure or a very high red blood cell count, and men trying to conceive now. Dr. Ewals goes through all of this before anything is prescribed.

Can testosterone treatment affect fertility?

Yes. Testosterone therapy can lower sperm production. If you might want children, say so at your first visit so you can discuss other options.

I'm already on testosterone elsewhere. Can I switch to you?

Yes. Email or fax your most recent labs and your last visit note (info@alternativehealthgf.com or (701) 757-0974), then call Will to book. Dr. Ewals reviews your records first. Depending on what they show, she may order additional testing before continuing your treatment.

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